Architectural asymmetry enables DNA transport through the Helicobacter pylori cag type IV secretion system.

ABSTRACT: Structural asymmetry within secretion system architecture is fundamentally important for apparatus diversification and biological function. However, the mechanism by which symmetry mismatch contributes to nanomachine assembly and interkingdom effector translocation are undefined. Here, we show that architectural asymmetry orchestrates dynamic substrate selection and enables trans-kingdom DNA conjugation through the Helicobacter pylori cag type IV secretion system (cag T4SS). Structural analyses of asymmetric units within the cag T4SS periplasmic ring complex (PRC) revealed intermolecular π-π stacking interactions that coordinate DNA binding and license trans-kingdom conjugation without disrupting the translocation of protein and peptidoglycan effector molecules. Additi

SUBMITTER: Ryan ME 

PROVIDER: S-EPMC10402047 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature