The Role of the Tyrosine-Based Sorting Signals of the ORF3a Protein of SARS-CoV-2 on Intracellular Trafficking, Autophagy, and Apoptosis.

ABSTRACT: The open reading frame 3a (ORF3a) is an accessory transmembrane protein that is important to the pathogenicity of SARS-CoV-2. The cytoplasmic domain of ORF3a has three canonical tyrosine-based sorting signals (YxxΦ; where x is any amino acid and Φ is a hydrophobic amino acid with a bulky -R group). They have been implicated in the trafficking of membrane proteins to the cell plasma membrane and to intracellular organelles. Previous studies have indicated that mutation of the ¹⁶⁰YSNV¹⁶³ motif abrogated plasma membrane expression and inhibited ORF3a-induced apoptosis. However, two additional canonical tyrosine-based sorting motifs (²¹¹YYQL²¹³, ²³³YNKI²³⁶) exist in the cytoplasmic domain of ORF3a that have not been assessed. We

SUBMITTER: Henke W 

PROVIDER: S-EPMC10402054 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature