Automatic design of gene regulatory mechanisms for spatial pattern formation.

ABSTRACT: Synthetic developmental biology aims to engineer gene regulatory mechanisms (GRMs) for understanding and producing desired multicellular patterns and shapes. However, designing GRMs for spatial patterns is a current challenge due to the nonlinear interactions and feedback loops in genetic circuits. Here we present a methodology to automatically design GRMs that can produce any given spatial pattern. The proposed approach uses two orthogonal morphogen gradients acting as positional information signals in a multicellular tissue area or culture, which constitutes a continuous field of engineered cells implementing the same designed GRM. To efficiently design both the circuit network and the interaction mechanisms-including the number of genes necessary for the formation of the target pattern-

SUBMITTER: Mousavi R 

PROVIDER: S-EPMC10402059 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature