Genome-scale chromatin interaction dynamic measurements for key components of the RNA Pol II general transcription machinery.

ABSTRACT:

Background

A great deal of work has revealed in structural detail the components of the machinery responsible for mRNA gene transcription initiation. These include the general transcription factors (GTFs), which assemble at promoters along with RNA Polymerase II (Pol II) to form a preinitiation complex (PIC) aided by the activities of cofactors and site-specific transcription factors (TFs). However, less well understood are the in vivo PIC assembly pathways and their kinetics, an understanding of which is vital for determining on a mechanistic level how rates of in vivo RNA synthesis are established and how cofactors and TFs impact them.

Results

We used competition ChIP to obtain genome-scale estimates of the residence times for five GTFs: TBP, TFIIA, TFIIB, TF

SUBMITTER: Kupkova K 

PROVIDER: S-EPMC10402067 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature