Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles.

ABSTRACT: Microglia are resident immune cells of the brain that play important roles in mediating inflammatory responses in several neurological diseases via direct and indirect mechanisms. One indirect mechanism may involve extracellular vesicle (EV) release, so that the molecular cargo transported by microglia-derived EVs can have functional effects by facilitating intercellular communication. The molecular composition of microglia-derived EVs, and how microglial activation states impacts EV composition and EV-mediated effects in neuroinflammation, remain poorly understood. We hypothesize that microglia-derived EVs have unique molecular profiles that are determined by microglial activation state. Using size-exclusion chromatography to purify EVs from BV2 microglia, combined with proteomic (label-f

SUBMITTER: Santiago JV 

PROVIDER: S-EPMC10402142 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature