Cross-site reproducibility of human cortical organoids reveals consistent cell type composition and architecture.

ABSTRACT:

Background

Reproducibility of human cortical organoid (hCO) phenotypes remains a concern for modeling neurodevelopmental disorders. While guided hCO protocols reproducibly generate cortical cell types in multiple cell lines at one site, variability across sites using a harmonized protocol has not yet been evaluated. We present an hCO cross-site reproducibility study examining multiple phenotypes.

Methods

Three independent research groups generated hCOs from one induced pluripotent stem cell (iPSC) line using a harmonized miniaturized spinning bioreactor protocol. scRNA-seq, 3D fluorescent imaging, phase contrast imaging, qPCR, and flow cytometry were used to characterize the 3 month differentiations across sites.

Results

In all sites, hCOs were mostly cortical progeni

SUBMITTER: Glass MR 

PROVIDER: S-EPMC10402155 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature