Quercetin improves epithelial regeneration from airway basal cells of COPD patients.

ABSTRACT:

Background

Airway basal cells from patients with chronic obstructive pulmonary disease (COPD) regenerate abnormal airway epithelium and this was associated with reduced expression of several genes involved in epithelial repair. Quercetin reduces goblet cell metaplasia and the expression of pro-inflammatory cytokines in COPD models. This study assessed whether quercetin improves epithelial regeneration from COPD airway basal cells.

Methods

COPD airway basal cells were treated with DMSO or 1 μM quercetin for three days. The cells were then cultured at air/liquid interface (ALI) for up to 4 weeks. Basal cells from healthy donors cultured at air/liquid interface were used as controls. Polarization of cells was determined at 8 days of ALI. The cell types and IL-8 expression in di

SUBMITTER: McCluskey ES 

PROVIDER: S-EPMC10402257 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature