Assessing and harnessing updated polyketide synthase modules through combinatorial engineering.

ABSTRACT: The modular nature of polyketide assembly lines and the significance of their products make them prime targets for combinatorial engineering. While short synthases constructed using the recently updated module boundary have been shown to outperform those using the traditional boundary, larger synthases constructed using the updated boundary have not been investigated. Here we describe our design and implementation of a BioBricks-like platform to rapidly construct 5 triketide, 25 tetraketide, and 125 pentaketide synthases from the updated modules of the Pikromycin synthase. Every combinatorial possibility of modules 2-6 inserted between the first and last modules of the native synthase was constructed and assayed. Anticipated products were observed from 60% of the triketide synthases, 32% o

SUBMITTER: Ray KA 

PROVIDER: S-EPMC10402262 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature