Pre-culture with transferrin-Fe³⁺ before in vitro maturation improves the developmental competence of porcine oocytes matured in vitro.

ABSTRACT:

Purpose

Since the developmental competence of oocytes cultured after in vitro maturation (IVM) is low, it is necessary to improve the IVM method for efficient offspring production. In this study, we revealed that transferrin (TF)-Fe³⁺ was accumulated in follicular fluid with increasing the follicular diameter, and that TF receptor (TFR1) was localized in granulosa cells of pig. Thus, we hypothesized that TF-Fe³⁺ would be a factor in the induction of developmental competence of porcine oocytes.

Methods

To mimic the follicular development environment, cumulus-oocyte complexes (COCs) were cultured in pre-IVM medium (low dose of FSH) without or with Holo-TF (monoferric or diferric TF) or Apo-TF (non-iron bond TF). After pre-IVM without or with Holo-TF, COCs

SUBMITTER: Tonai S 

PROVIDER: S-EPMC10402462 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature