Validation of a metabolite-GWAS network for Populus trichocarpa family 1 UDP-glycosyltransferases.

ABSTRACT: Metabolite genome-wide association studies (mGWASs) are increasingly used to discover the genetic basis of target phenotypes in plants such as Populus trichocarpa, a biofuel feedstock and model woody plant species. Despite their growing importance in plant genetics and metabolomics, few mGWASs are experimentally validated. Here, we present a functional genomics workflow for validating mGWAS-predicted enzyme-substrate relationships. We focus on uridine diphosphate-glycosyltransferases (UGTs), a large family of enzymes that catalyze sugar transfer to a variety of plant secondary metabolites involved in defense, signaling, and lignification. Glycosylation influences physiological roles, localization within cells and tissues, and metabolic fates of these metabolites. UGTs have substanti

SUBMITTER: Saint-Vincent PMB 

PROVIDER: S-EPMC10402742 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature