Arginine regulates HSPA5/BiP translation through ribosome pausing in triple-negative breast cancer cells.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with a high mortality rate due to a lack of therapeutic targets. Many TNBC cells are reliant on extracellular arginine for survival and express high levels of binding immunoglobin protein (BiP), a marker of metastasis and endoplasmic reticulum (ER) stress response.

Methods

In this study, the effect of arginine shortage on BiP expression in the TNBC cell line MDA-MB-231 was evaluated. Two stable cell lines were generated in MDA-MB-231 cells: the first expressed wild-type BiP, and the second expressed a mutated BiP free of the two arginine pause-site codons, CCU and CGU, termed G-BiP.

Results

The results showed that arginine shortage induced a non-canonical ER stress response by in

SUBMITTER: Vidal CM 

PROVIDER: S-EPMC10403569 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature