Large-scale reference-free analysis of flavivirus sequences in Aedes aegypti whole genome DNA sequencing data.

ABSTRACT: Flaviviruses are a diverse group of RNA viruses, which include the etiological agents of Zika, dengue and yellow fever that are transmitted by mosquitoes. Flaviviruses do not encode reverse transcriptase and cannot reverse transcribe into DNA, yet DNA sequences of flaviviruses are found both integrated in the chromosomes of Aedes aegypti mosquitoes and as extrachromosomal sequences. We have previously examined the Ae. aegypti reference genome to identify flavivirus integrations and analyzed conservation of these sequences among whole-genome data of 464 Ae. aegypti collected across 10 countries globally. Here, we extended this analysis by identifying flavivirus sequences in these samples independently of the Ae. aegypti reference assembly. Our aim was to identify the complete set of viral s

SUBMITTER: Spadar A 

PROVIDER: S-EPMC10403824 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature