FACT regulates pluripotency through proximal and distal regulation of gene expression in murine embryonic stem cells.

ABSTRACT:

Background

The FACT complex is a conserved histone chaperone with critical roles in transcription and histone deposition. FACT is essential in pluripotent and cancer cells, but otherwise dispensable for most mammalian cell types. FACT deletion or inhibition can block induction of pluripotent stem cells, yet the mechanism through which FACT regulates cell fate decisions remains unclear.

Results

To explore the mechanism for FACT function, we generated AID-tagged murine embryonic cell lines for FACT subunit SPT16 and paired depletion with nascent transcription and chromatin accessibility analyses. We also analyzed SPT16 occupancy using CUT&RUN and found that SPT16 localizes to both promoter and enhancer elements, with a strong overlap in binding with OCT4, SOX2, and NANOG. Over

SUBMITTER: Klein DC 

PROVIDER: S-EPMC10403911 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature