Oral nicotine pouches with an aftertaste? Part 2: in vitro toxicity in human gingival fibroblasts.

ABSTRACT: Nicotine pouches contain fewer characteristic toxicants than conventional tobacco products. However, the associated risks in terms of toxicity and addiction potential are still unclear. Therefore, endpoints of toxicity and contents of flavoring substances were investigated in this study. The in vitro toxicity of five different nicotine pouches and the reference snus CRP1.1 were studied in human gingival fibroblasts (HGF-1). Cells were exposed to product extracts (nicotine contents: 0.03-1.34 mg/mL) and sampled at different time points. Cytotoxicity, total cellular reactive oxygen species (ROS) levels, and changes in the expression levels of inflammatory and oxidative stress genes were assessed. Flavor compounds used in the nicotine pouches were identified by GC-MS. Cytotoxicity was observe

SUBMITTER: Rinaldi S 

PROVIDER: S-EPMC10404181 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature