The splicing factor YBX1 promotes the progression of osteosarcoma by upregulating VEGF₁₆₅ and downregulating VEGF_165b.

ABSTRACT: VEGF₁₆₅ and its isoform VEGF_165b have the same length but opposite functions in cancer. Some studies have indicated the important role of VEGF₁₆₅ in osteosarcoma (OS); however, VEGF_165b has not been taken into consideration. This study aims to clarify the roles of the two isoforms in OS and the mechanism controlling their formation from an alternative splicing perspective. By in vivo and in vitro experiments, we assessed the expression and function of VEGF₁₆₅ and VEGF_165b, screened the underlying splicing factors, and verified the regulatory function of splicing factor YBX1 on the two isoforms and its role in OS. The results showed that in OS, VEGF₁₆₅ was upregulated but VEGF_165b was downregulated. VEGF

SUBMITTER: Quan B 

PROVIDER: S-EPMC10405001 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature