Depletion of soluble cytokines unlocks the immunomodulatory bioactivity of extracellular vesicles.

ABSTRACT: Despite an enormous interest in understanding the bioactivity of extracellular vesicles (EV) in physiology and disease for the development of therapeutic applications, the impact of EV preparation methods remains minimally explored. In this study, we implemented density gradient ultracentrifugation combined with size-exclusion chromatography (DG-SEC), differential ultracentrifugation (dUC) and/or stand-alone SEC (sSEC) to fractionate media conditioned by different cancer cells and/or cancer-associated fibroblasts (CAF). EV-enriched but protein-depleted versus EV-depleted but protein-enriched DG-SEC fractions, and EV-containing dUC and sSEC preparations were quality controlled for particle number, protein concentration, selected protein composition and ultrastructure, characterized for thei

SUBMITTER: Roux Q 

PROVIDER: S-EPMC10405237 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature