Thymosin β4 Alleviates Autoimmune Dacryoadenitis via Suppressing Th17 Cell Response.

ABSTRACT:

Purpose

We investigated the therapeutic effect of recombinant thymosin β4 (rTβ4) on rabbit autoimmune dacryoadenitis, an animal model of SS dry eye, and explore its mechanisms.

Methods

Rabbits were treated topically with rTβ4 or PBS solution after disease onset for 28 days, and clinical scores were determined by assessing tear secretion, break-up time, fluorescein, hematoxylin and eosin staining, and periodic acid-Schiff. The expression of inflammatory mediators in the lacrimal glands were measured by real-time PCR. The expression of T helper 17 (Th17) cell-related transcription factors and cytokines were detected by real-time PCR and Western blotting. The molecular mechanism underlying the effects of rTβ4 on Th17 cell responses was investigated by Western blotting.

Resul

SUBMITTER: Zhao X 

PROVIDER: S-EPMC10405860 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature