Imaging mitochondria through bone in live mice using two-photon fluorescence microscopy with adaptive optics.

ABSTRACT:

Introduction

Mitochondria are extremely important organelles in the regulation of bone marrow and brain activity. However, live imaging of these subcellular features with high resolution in scattering tissues like brain or bone has proven challenging.

Methods

In this study, we developed a two-photon fluorescence microscope with adaptive optics (TPFM-AO) for high-resolution imaging, which uses a home-built Shack-Hartmann wavefront sensor (SHWFS) to correct system aberrations and a sensorless approach for correcting low order tissue aberrations.

Results

Using AO increases the fluorescence intensity of the point spread function (PSF) and achieves fast imaging of subcellular organelles with 400 nm resolution through 85 μm of highly scattering tissue. We achieved ~1.55×, ~

SUBMITTER: Zheng T 

PROVIDER: S-EPMC10406258 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature