Gluconeogenesis in the Yolk Sac Membrane: Enzyme Activity, Gene Expression, and Metabolites During Layer Chicken Development.

ABSTRACT: Yolk sac membranes of layer eggs were collected daily (n = 7-9) from day three of incubation to day three post-hatch, and mRNA expression and activities were quantified for key gluconeogenesis enzymes (glucose-6-phosphatase, fructose-1,6-bisphosphatase, cytosolic and mitochondrial phosphoenolpyruvate carboxykinases, and pyruvate carboxylase). Lactate, triglycerides, non-esterified fatty acids, glycogen, and glucose in the yolk sac membrane, and blood glucose levels were also measured. The mRNA expression and activity were detected for all enzymes. Differences in expression levels and enzyme activities seemed to reflect the embryo's developmental environment and physiological demands at different developmental stages. During the first week to the mid-second week of incubation, the expressio

SUBMITTER: Shibata M 

PROVIDER: S-EPMC10406515 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature