Metabonomic analysis of seminal plasma in necrozoospermia patients based on liquid chromatography-mass spectrometry.

ABSTRACT:

Background

In the pathological study of necrozoospermia-a form of sperm mortality-the underlying metabolic mechanism remains unclear. Thus, the aim of this study was to characterize metabolic alterations in the seminal plasma of necrozoospermic patients and to provide insights into the etiology of the disease.

Methods

Seminal plasma samples were collected from patients diagnosed with necrozoospermia (n=28) as well as normozoospermia (n=37). The samples were analyzed using nontargeted metabolomics based on liquid chromatography-mass spectrometry (LC-MS). The raw data were subjected to multivariate analysis to identify metabolites correlated with necrozoospermia. Differential metabolites were subjected to pathway analysis using MetaboAnalyst.

Results

The results of the

SUBMITTER: Deng T 

PROVIDER: S-EPMC10406541 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature