Focal-adhesion kinase regulates the sialylation of N-glycans via the PI4KIIα-PI4P pathway.

ABSTRACT: Sialylation is a terminal glycosylated modification of glycoproteins that regulates critical biological events such as cell adhesion and immune response. Our previous study showed that integrin α3β1 plays a crucial role in regulating the sialylation of N-glycans. However, the underlying mechanism for the regulation remains unclear. This study investigated how sialylation is affected by focal adhesion kinase (FAK), which is a critical downstream signal molecule of integrin β1. We established a stable FAK knockout (KO) cell line using the CRISPR/Cas9 system in HeLa cells. The results obtained from lectin blot, flow cytometric analysis, and MS showed that the sialylation levels were significantly decreased in the KO cells compared with that in wild-type (WT) cells. Moreover, phosphatidylinosi

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10406863 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature