A fluorometric assay to determine labile copper(II) ions in serum.

ABSTRACT: Labile copper(II) ions (Cu²⁺) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu²⁺ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson's disease (WD) or neurological disorders. So far, no direct quantification method has been described to determine this small Cu²⁺ subset. This study introduces a fluorometric high throughput assay using the novel Cu²⁺ binding fluoresceine-peptide sensor FP4 (Kd of the Cu²⁺-FP4-complex 0.38 pM) to determine labile Cu²⁺ in human and rat serum. Using 96 human serum samples, labile Cu²⁺was measured to be 0.14 ± 0.05 pM, showing no correlation with age or other serum

SUBMITTER: Maares M 

PROVIDER: S-EPMC10406877 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature