Noxious chemical discrimination by Tribolium castaneum TRPA1 channel in the HEK293 cell expression system.

ABSTRACT: Nociception is the sensory perception of noxious chemical stimuli. Repellent behavior to avoid noxious stimuli is indispensable for survival, and this mechanism has been evolutionarily conserved across a wide range of species, from mammals to insects. The transient receptor potential ankyrin 1 (TRPA1) channel is one of the most conserved noxious chemical sensors. Here, we describe the heterologous stable expression of Tribolium castaneum TRPA1 (TcTRPA1) in human embryonic kidney (HEK293) cells. The intracellular Ca²⁺ influx was measured when two compounds, citronellal and l-menthol, derived from plant essential oils, were applied in vitro using a fluorescence assay. The analysis revealed that citronellal evoked Ca²⁺ influx dose-dependently for TcT

SUBMITTER: Shimomura K 

PROVIDER: S-EPMC10407194 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature