EndoC-βH5 cells are storable and ready-to-use human pancreatic beta cells with physiological insulin secretion.

ABSTRACT:

Objectives

Readily accessible human pancreatic beta cells that are functionally close to primary adult beta cells are a crucial model to better understand human beta cell physiology and develop new treatments for diabetes. We here report the characterization of EndoC-βH5 cells, the latest in the EndoC-βH cell family.

Methods

EndoC-βH5 cells were generated by integrative gene transfer of immortalizing transgenes hTERT and SV40 large T along with Herpes Simplex Virus-1 thymidine kinase into human fetal pancreas. Immortalizing transgenes were removed after amplification using CRE activation and remaining non-excized cells eliminated using ganciclovir. Resulting cells were distributed as ready to use EndoC-βH5 cells. We performed transcriptome, immunological and extensive functi

SUBMITTER: Blanchi B 

PROVIDER: S-EPMC10407753 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature