Droplet digital PCR as a tool to detect resistant isolates of Dirofilaria immitis.

ABSTRACT: Prevention of canine heartworm disease, caused by Dirofilaria immitis, relies on macrocyclic lactones for which drug resistance is now a concern. Although genetic polymorphisms have been associated with resistance in D. immitis populations, the mechanism is still not well understood. The lack of reliable in vitro assays to detect resistance is a limitation for confirming resistance. Ten single nucleotide polymorphisms (SNPs) were previously clinically validated in D. immitis resistant isolates, using the MiSeq platform. This technique although useful for research studies is expensive and does not facilitate rapid detection of these markers in small numbers of clinical samples. We developed a droplet digital PCR protocol for detecting SNPs correlating with ML resistance. Specific primers an

SUBMITTER: Kumar S 

PROVIDER: S-EPMC10407818 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature