ProSight Native: Defining Protein Complex Composition from Native Top-Down Mass Spectrometry Data.

ABSTRACT: Native mass spectrometry has recently moved alongside traditional structural biology techniques in its ability to provide clear insights into the composition of protein complexes. However, to date, limited software tools are available for the comprehensive analysis of native mass spectrometry data on protein complexes, particularly for experiments aimed at elucidating the composition of an intact protein complex. Here, we introduce ProSight Native as a start-to-finish informatics platform for analyzing native protein and protein complex data. Combining mass determination via spectral deconvolution with a top-down database search and stoichiometry calculations, ProSight Native can determine the complete composition of protein complexes. To demonstrate its features, we used ProSight Native t

SUBMITTER: Durbin KR 

PROVIDER: S-EPMC10407923 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature