Rhox6 regulates the expression of distinct target genes to mediate mouse PGCLC formation and ESC self-renewal.

ABSTRACT:

Background

Mouse embryonic stem cells (mESCs) not only retain the property of self-renewal but also have the ability to develop into primordial germ cell-like cells (PGCLCs). However, knowledge about the mechanisms of transcriptional regulation is still limited. Rhox6, a member of the homeobox family that is located on the X chromosome, is highly expressed within PGCLCs in vivo and in vitro. However, the detailed effects of Rhox6 on PGCLC specification and mESC maintenance remain unclear.

Results

In this study, we found that overexpression of Rhox6 favors the formation of PGCLCs, while depletion of Rhox6 inhibits the generation of PGCLCs. Mechanistically, Rhox6 directly induces the expression of Nanos3 during the specification of PGCLCs. Subsequently, downregulation of Nanos

SUBMITTER: Li X 

PROVIDER: S-EPMC10408072 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature