Generation of a recombinant Saffold Virus expressing UnaG as a marker for the visualization of viral infection.

ABSTRACT:

Background

Saffold virus (SAFV), which belongs to the genus Cardiovirus of the family Picornaviridae, is associated with acute respiratory or gastrointestinal illnesses in children; it is also suspected to cause severe diseases, such as acute flaccid paralysis and aseptic meningitis. However, the understanding of the mechanism of its pathogenicity is still limited due to the many unknowns about its lifecycle; for example, the cellular receptor for its infection remains to be determined. A system to monitor SAFV infection in vitro and in vivo is required in order to accelerate research on SAFV.

Results

We generated a recombinant SAFV expressing green fluorescent protein (GFP) or UnaG, a novel fluorescent protein derived from Japanese eel. HeLa cells infected by either GFP or

SUBMITTER: Okuwa T 

PROVIDER: S-EPMC10408109 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature