CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis.

ABSTRACT:

Background

Disease molecular complexity requires high throughput workflows to map disease pathways through analysis of vast tissue repositories. Great progress has been made in tissue multiomics analytical technologies. To match the high throughput of these advanced analytical platforms, we have previously developed a multipurpose 96-well microplate sonicator, PIXUL, that can be used in multiple workflows to extract analytes from cultured cells and tissue fragments for various downstream molecular assays. And yet, the sample preparation devices, such as PIXUL, along with the downstream multiomics analytical capabilities have not been fully exploited to interrogate tissues because storing and sampling of such biospecimens remain, in comparison, inefficient.

Results

To mitigat

SUBMITTER: Schactler SA 

PROVIDER: S-EPMC10408117 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature