Principal Component Analysis of 1D 1H Diffusion Edited NMR Spectra of Protein Therapeutics.

ABSTRACT: The one-dimensional (1D) diffusion edited proton NMR method, Protein Fingerprint by Lineshape Enhancement (PROFILE) has been demonstrated to be suitable for higher order structure (HOS) characterization of protein therapeutics including monoclonal antibodies. Recent reports in the literature have demonstrated its advantages for HOS characterization over traditional methods such as circular dichroism and Fourier-transform infrared spectroscopy. Previously, we have demonstrated that the PROFILE method is complementary with high resolution 2D methyl correlated NMR methods and how both may be deployed as a multi-modal platform to further the utility of NMR for HOS characterization. A major limitation of the PROFILE method remains its need for high signal to noise data due to its reliance on co

SUBMITTER: Elliott KW 

PROVIDER: S-EPMC10408412 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature