Expression properties, structural features and functional analysis of CALR E381A in MPN patients.

ABSTRACT:

Objective

To investigate the expression properties, structural features and function of CALR E381A in myeloproliferative neoplasms (MPN) patients.

Methods

In this retrospective study, 435 MPN patients admitted to the Department of Hematology, Ningbo First Hospital from July 2015 to July 2021 were selected as the study subjects. Mutations in CALR exon 9 from genomic DNA samples were identified by PCR, followed by Sanger sequencing. The physicochemical properties of the wild-type calreticulin and the p.E381A variant, and the structural information of the p.E381A variant were analyzed by using the bioinformatics databases. Growth assay of UT-7/mpl cells with CALR E381A was used for the functional analysis of CALR E381A.

Results

The predominant types of C

SUBMITTER: Xu K 

PROVIDER: S-EPMC10408505 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature