Transgene-free direct conversion of murine fibroblasts into functional muscle stem cells.

ABSTRACT: Transcription factor-based cellular reprogramming provides an attractive approach to produce desired cell types for regenerative medicine purposes. Such cellular conversions are widely dependent on viral vectors to efficiently deliver and express defined factors in target cells. However, use of viral vectors is associated with unfavorable genomic integrations that can trigger deleterious molecular consequences, rendering this method a potential impediment to clinical applications. Here, we report on a highly efficient transgene-free approach to directly convert mouse fibroblasts into induced myogenic progenitor cells (iMPCs) by overexpression of synthetic MyoD-mRNA in concert with an enhanced small molecule cocktail. First, we performed a candidate compound screen and identified two molecu

SUBMITTER: Qabrati X 

PROVIDER: S-EPMC10409758 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature