Experimental design considerations for studies of human tear proteins.

ABSTRACT:

Purpose

Human tears contain abundant, diverse sets of proteins that may serve as biomarkers of ocular surface health. There is a need for reproducible methods that consider multiple factors influencing the tear proteome, in addition to the variable of interest. Here we examined a workflow for proteomic analysis of tear proteins without the need to pool tear samples from multiple individuals, thus allowing for analyses based on individual factors, and increasing opportunities for protein biomarker discovery.

Methods

Tears were collected by Schirmer strip following topical ocular anesthetic application then individually stored at -80 °C prior to processing for proteomics. Tear proteins were extracted from Schirmer strips, digested using suspension trapping spin columns (S-Trap

SUBMITTER: Harkness BM 

PROVIDER: S-EPMC10409878 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature