Assessment of antibodies in the upper and lower human respiratory tract at steady state and after respiratory viral infection.

ABSTRACT:

Objectives

There is an increasing appreciation for the need to study mucosal antibody responses in humans. Our aim was to determine the utility of different types of samples from the human respiratory tract, specifically nasopharyngeal (NP) swabs obtained for diagnostic purposes and bronchoalveolar lavage (BAL) obtained in outpatient and inpatient settings.

Methods

We analysed antibody levels in plasma and NP swabs from 67 individuals with acute influenza as well as plasma and BAL from individuals undergoing bronchoscopy, including five control subjects as well as seven moderately and seven severely ill subjects with a respiratory viral infection. Levels of α2-macroglobulin were determined in BAL and plasma to assess plasma exudation.

Results

IgG and IgA were readily

SUBMITTER: Koutsakos M 

PROVIDER: S-EPMC10410120 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature