Nanopore sequencing unveils the complexity of the cold-activated murine brown adipose tissue transcriptome.

ABSTRACT: Alternative transcription increases transcriptome complexity by expression of multiple transcripts per gene. Annotation and quantification of transcripts using short-read sequencing is non-trivial. Long-read sequencing aims at overcoming these problems by sequencing full-length transcripts. Activation of brown adipose tissue (BAT) thermogenesis involves major transcriptomic remodeling and positively affects metabolism via increased energy expenditure. We benchmark Oxford Nanopore Technology (ONT) long-read sequencing protocols to Illumina short-read sequencing assessing alignment characteristics, gene and transcript detection and quantification, differential gene and transcript expression, transcriptome reannotation, and differential transcript usage (DTU). We find ONT sequencing is superi

SUBMITTER: Engelhard CA 

PROVIDER: S-EPMC10410515 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature