Library preparation method and DNA source influence endogenous DNA recovery from 100-year-old avian museum specimens.

ABSTRACT: Museum specimens collected prior to cryogenic tissue storage are increasingly being used as genetic resources, and though high-throughput sequencing is becoming more cost-efficient, whole genome sequencing (WGS) of historical DNA (hDNA) remains inefficient and costly due to its short fragment sizes and high loads of exogenous DNA, among other factors. It is also unclear how sequencing efficiency is influenced by DNA sources. We aimed to identify the most efficient method and DNA source for collecting WGS data from avian museum specimens. We analyzed low-coverage WGS from 60 DNA libraries prepared from four American Robin (Turdus migratorius) and four Abyssinian Thrush (Turdus abyssinicus) specimens collected in the 1920s. We compared DNA source (toepad versus incision-line sk

SUBMITTER: Settlecowski AE 

PROVIDER: S-EPMC10410627 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature