Macrophage-organoid co-culture model for identifying treatment strategies against macrophage-related gemcitabine resistance.

ABSTRACT:

Background

Gemcitabine resistance (GR) is a significant clinical challenge in pancreatic adenocarcinoma (PAAD) treatment. Macrophages in the tumor immune-microenvironment are closely related to GR. Uncovering the macrophage-induced GR mechanism could help devise a novel strategy to improve gemcitabine treatment outcomes in PAAD. Therefore, preclinical models accurately replicating patient tumor properties are essential for cancer research and drug development. Patient-derived organoids (PDOs) represent a promising in vitro model for investigating tumor targets, accelerating drug development, and enabling personalized treatment strategies to improve patient outcomes.

Methods

To investigate the effects of macrophage stimulation on GR, co-cultures were set up using PDOs from th

SUBMITTER: Jiang S 

PROVIDER: S-EPMC10411021 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature