Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.

ABSTRACT:

Objective

To use trophoblast cells accumulating in the endocervical canal at the beginning of pregnancy for noninvasive prenatal testing.

Design

Prospective, double-blinded test for fetal gender.

Setting

Academic medical center.

Patient(s)

Fifty-six women with singleton pregnancies at gestational age 5-20 weeks.

Intervention(s)

Isolation of fetal cells from resident maternal cells in endocervical specimens using anti-human leukocyte antigen G coupled to magnetic nanoparticles; cell phenotyping immunofluorescently with a panel of trophoblast subtype-specific proteins; DNA integrity assessment with terminal dUTP nick-end labeling (TUNEL); and polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) to detect sex chromosomes in individua

SUBMITTER: Bolnick JM 

PROVIDER: S-EPMC10411519 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature