Molecular mechanisms regulating natural menopause in the female ovary: a study based on transcriptomic data.

ABSTRACT:

Introduction

Natural menopause is an inevitable biological process with significant implications for women's health. However, the molecular mechanisms underlying menopause are not well understood. This study aimed to investigate the molecular and cellular changes occurring in the ovary before and after perimenopause.

Methods

Single-cell sequencing data from the GTEx V8 cohort (30-39: 14 individuals; 40-49: 37 individuals; 50-59: 61 individuals) and transcriptome sequencing data from ovarian tissue were analyzed. Seurat was used for single-cell sequencing data analysis, while harmony was employed for data integration. Cell differentiation trajectories were inferred using CytoTrace. CIBERSORTX assessed cell infiltration scores in ovarian tissue. WGCNA evaluated co-expression n

SUBMITTER: Liu Q 

PROVIDER: S-EPMC10411606 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature