Chemical sensors for imaging total cellular aliphatic aldehydes in live cells.

ABSTRACT: Aliphatic aldehydes are reactive electrophilic carbonyls that cross-link with DNA and proteins leading to cellular toxicity and disease pathogenesis. This toxicity is due to the cooperative effect of multiple aldehydes via a common mechanism. Therefore, live-cell imaging of total aliphatic aldehydes, small-to-long chain (C₁-C₁₀), is highly desired to decipher their physiological and pathological functions. However, sensors for imaging total cellular aliphatic aldehydes are currently lacking despite their high concentrations (∼80 to >500 μM) inside cells. Herein, we report chemical sensors that generate a benzimidazole moiety upon reaction with aliphatic aldehydes of different chain lengths (C₁-C₁₀), resulting in turn-on fluorescence. These

SUBMITTER: Wills R 

PROVIDER: S-EPMC10411626 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature