Genetically targeted chemical assembly of polymers specifically localized extracellularly to surface membranes of living neurons.

ABSTRACT: Multicellular biological systems, particularly living neural networks, exhibit highly complex organization properties that pose difficulties for building cell-specific biocompatible interfaces. We previously developed an approach to genetically program cells to assemble structures that modify electrical properties of neurons in situ, opening up the possibility of building minimally invasive cell-specific structures and interfaces. However, the efficiency and biocompatibility of this approach were challenged by limited membrane targeting of the constructed materials. Here, we design a method for highly localized expression of enzymes targeted to the plasma membrane of primary neurons, with minimal intracellular retention. Next, we show that polymers synthesized in situ by this approach form

SUBMITTER: Zhang A 

PROVIDER: S-EPMC10411876 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature