Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3',5'-cyclic monophosphate signaling.

ABSTRACT:

Objective

To determine the effect of sildenafil, a phosphodiesterase type 5 inhibitor, on trophoblast invasiveness.

Design

Laboratory investigation.

Setting

Academic medical center.

Patient(s)

Placental tissues discarded after first-trimester terminations were obtained from patients with informed consent.

Intervention(s)

A cell line, HTR-8/SVneo, established from first-trimester cytotrophoblast, and villous explants, was treated with or without sildenafil, guanosine 3',5'-cyclic monophosphate (cGMP) analog, cGMP inhibitor, or L-NAME (N(G)-nitro-L-arginine methyl ester hydrochloride) and cultured on fibronectin or Matrigel. Integrins α6β4 and α1β1 were detected by immunocytochemistry.

Main outcome measure(s)

Trophoblast outgrowth from villous tips,

SUBMITTER: Bolnick JM 

PROVIDER: S-EPMC10411948 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature