Insights into the protonation state and spin structure for the g = 2 multiline electron paramagnetic resonance signal of the oxygen-evolving complex.

ABSTRACT: In photosystem II (PSII), one-electron oxidation of the most stable oxidation state of the Mn₄CaO₅ cluster (S₁) leads to formation of two distinct states, the open-cubane S₂ conformation [Mn1(III)Mn2(IV)Mn3(IV)Mn4(IV)] with low spin and the closed-cubane S₂ conformation [Mn1(IV)Mn2(IV)Mn3(IV)Mn4(III)] with high spin. In electron paramagnetic resonance (EPR) spectroscopy, the open-cubane S₂ conformation exhibits a g = 2 multiline signal. However, its protonation state remains unclear. Here, we investigated the protonation state of the open-cubane S₂ conformation by calculating exchange couplings in the presence of the PSII protein environment and simulating the pulsed electron-electron double resonance (PELD

SUBMITTER: Saito K 

PROVIDER: S-EPMC10411963 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature