Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury.

ABSTRACT: Spinal cord injury (SCI) leads to severe sensory and motor dysfunction below the lesion. However, the cellular dynamic responses and heterogeneity across different regions below the lesion remain to be elusive. Here, we used single-cell transcriptomics to investigate the region-related cellular responses in female rhesus monkeys with complete thoracic SCI from acute to chronic phases. We found that distal lumbar tissue cells were severely impacted, leading to degenerative microenvironments characterized by disease-associated microglia and oligodendrocytes activation alongside increased inhibitory interneurons proportion following SCI. By implanting scaffold into the injury sites, we could improve the injury microenvironment through glial cells and fibroblast regulation while remodeling spa

SUBMITTER: Fan Y 

PROVIDER: S-EPMC10412553 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature