Low-dose radiation induces unstable gene expression in developing human iPSC-derived retinal ganglion organoids.

ABSTRACT: The effects of low-dose radiation on undifferentiated cells carry important implications. However, the effects on developing retinal cells remain unclear. Here, we analyzed the gene expression characteristics of neuronal organoids containing immature human retinal cells under low-dose radiation and predicted their changes. Developing retinal cells generated from human induced pluripotent stem cells (iPSCs) were irradiated with either 30 or 180 mGy on days 4-5 of development for 24 h. Genome-wide gene expression was observed until day 35. A knowledge-based pathway analysis algorithm revealed fluctuations in Rho signaling and many other pathways. After a month, the levels of an essential transcription factor of eye development, the proportion of paired box 6 (PAX6)-positive cells, and the pr

SUBMITTER: Katsura M 

PROVIDER: S-EPMC10412642 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature