Intrathecal [⁶⁴Cu]Cu-albumin PET reveals age-related decline of lymphatic drainage of cerebrospinal fluid.

ABSTRACT: Age-related cognitive decline is associated with dysfunctional lymphatic drainage of cerebrospinal fluid (CSF) through meningeal lymphatic vessels. In this study, intrathecal [⁶⁴Cu]Cu-albumin positron emission tomography (PET) was applied in mice to evaluate lymphatic drainage of CSF and its variation with age. [⁶⁴Cu]Cu-albumin PET was performed at multiple time points after intrathecal injection of [⁶⁴Cu]Cu-albumin at an infusion rate of 700 nl/min in adult and aged mice (15-25 months old). CSF clearance and paravertebral lymph nodes were quantified after injection and during the stationary phase. Stationary phase of the next day followed the initial perturbed state by injection of 6 ul (1/7 of total CSF volume) and CSF clearance half-time from the subarac

SUBMITTER: Sarker A 

PROVIDER: S-EPMC10412645 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature