Cytosolic Ca²⁺ gradients and mitochondrial Ca²⁺ uptake in resting muscle fibers: A model analysis.

ABSTRACT: Calcium ions (Ca²⁺) enter mitochondria via the mitochondrial Ca²⁺ uniporter, driven by electrical and concentration gradients. In this regard, transgenic mouse models, such as calsequestrin knockout (CSQ-KO) mice, with higher mitochondrial Ca²⁺ concentrations ([Ca²⁺]_mito), should display higher cytosolic Ca²⁺ concentrations ([Ca²⁺]_cyto). However, repeated measurements of [Ca²⁺]_cyto in quiescent CSQ-KO fibers never showed a difference between WT and CSQ-KO. Starting from the consideration that fluorescent Ca²⁺ probes (Fura-2 and Indo-1) measure averaged global cytosolic concentrations, in this report we explored the role of local Ca²⁺ concentrations (i.e., Ca

SUBMITTER: Marcucci L 

PROVIDER: S-EPMC10412765 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature