A time-resolved clonogenic assay for improved cell survival and RBE measurements.

ABSTRACT:

Purpose

The in vitro clonogenic assay (IVCA) is the mainstay of quantitative radiobiology. Here, we investigate the benefit of a time-resolved IVCA version (trIVCA) to improve the quantification of clonogenic survival and relative biological effectiveness (RBE) by analyzing cell colony growth behavior.

Materials & methods

In the IVCA, clonogenicity classification of cell colonies is performed based on a fixed colony size threshold after incubation. In contrast, using trIVCA, we acquire time-lapse microscopy images during incubation and track the growth of each colony using neural-net-based image segmentation. Attributes of the resulting growth curves are then used as predictors for a decision tree classifier to determine clonogenicity of each colony. The method was applied t

SUBMITTER: Koch RA 

PROVIDER: S-EPMC10412889 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature