Heterogeneity in quiescent Muller glia in the uninjured zebrafish retina drive differential responses following photoreceptor ablation.

ABSTRACT:

Introduction

Loss of neurons in the neural retina is a leading cause of vision loss. While humans do not possess the capacity for retinal regeneration, zebrafish can achieve this through activation of resident Müller glia. Remarkably, despite the presence of Müller glia in humans and other mammalian vertebrates, these cells lack an intrinsic ability to contribute to regeneration. Upon activation, zebrafish Müller glia can adopt a stem cell-like state, undergo proliferation and generate new neurons. However, the underlying molecular mechanisms of this activation subsequent retinal regeneration remains unclear.

Methods/results

To address this, we performed single-cell RNA sequencing (scRNA-seq) and report remarkable heterogeneity in gene expression within quiescent Müller glia

SUBMITTER: Krylov A 

PROVIDER: S-EPMC10413128 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature