Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead.

ABSTRACT: With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue. The pbr operon from Klebsiella pneumoniae is selectively induced by bioavailable Pb(II). In the present study, the proviolacein biosynthetic gene cluster was transcriptionally fused to the pbr Pb(II) responsive element and introduced into Escherichia coli. The resultant biosensor responded to Pb(II) in a time- and dose-dependent manner. After a 5-h incubation wit

SUBMITTER: Zhu DL 

PROVIDER: S-EPMC10413148 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature